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Literature summary extracted from

  • Jung, J.; Nidetzky, B.
    Evidence of a sequestered imine intermediate during reduction of nitrile to amine by the nitrile reductase QueF from Escherichia coli (2018), J. Biol. Chem., 293, 3720-3733 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.13 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.7.1.13 E89A the variant performs primarily (more than 90%) a two-electron reduction of 7-cyano-7-deazaguanine (preQ0), releasing hydrolyzed imine (7-formyl-7-deazaguanine) as the product Escherichia coli
1.7.1.13 E89L the variant performs primarily (more than 90%) a two-electron reduction of 7-cyano-7-deazaguanine (preQ0), releasing hydrolyzed imine (7-formyl-7-deazaguanine) as the product Escherichia coli
1.7.1.13 F228A the variant performs primarily (more than 90%) a two-electron reduction of 7-cyano-7-deazaguanine (preQ0), releasing hydrolyzed imine (7-formyl-7-deazaguanine) as the product Escherichia coli
1.7.1.13 I192A the 7-cyano-7-deazaguanine (preQ0) reduction by the mutant gives 7-aminomethyl-7-deazaguanine (preQ1) and 7-formyl-7-deazaguanine at a 1:1 ratio Escherichia coli
1.7.1.13 L191A the 7-cyano-7-deazaguanine (preQ0) reduction by the mutant gives 7-aminomethyl-7-deazaguanine (preQ1) and 7-formyl-7-deazaguanine at a 4:1 ratio Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.13 7-cyano-7-deazaguanine + 2 NADPH + 2 H+ Escherichia coli i.e. preQ0 7-aminomethyl-7-deazaguanine + 2 NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.13 Escherichia coli Q46920
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.13 7-cyano-7-deazaguanine + 2 NADPH + 2 H+ i.e. preQ0 Escherichia coli 7-aminomethyl-7-deazaguanine + 2 NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.13 homodimer 2 * 71772, calculated from amino acid sequence Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.7.1.13 QueF
-
 Escherichia coli